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Based on our study, HIF-1α could be a potential therapeutic target for ischemic stroke.Ĭerebrovascular diseases, particularly ischemic stroke, have been widely studied because of their severe outcomes. Considerable reduction of blood to defective tissue hinders oxygen (O 2) and nutrient transportation, consequently causing cell death and tissue hypoxia.

To effectively deal with these harmful effects, the organ endeavors to augment oxygen supply to damaged tissues. Induction of angiogenesis is an effective pathway towards increasing the oxygenation of hypoxic tissue. In this context, hypoxia-inducible factor 1 (HIF-1α) can regulate the expression of numerous genes, and consequently enhance the adaptability to minimal O 2 environment. It guides the genes related to angiogenesis, vasomotor regulation, and metabolism processes. Such genes can remarkably facilitate neuronal cells regeneration after ischemia-induced brain damage. Previous studies reported that HIF-1α regulation and its accompanying impact on ischemic outcomes could be directed by the ischemic duration and degree of severity. Also, previous reports revealed that the HIF-1α stabilization usually occurs by inhibiting the prolyl-4-hydroxylase domain (PHD) activity, which directs the upregulation of vascular endothelial growth factor (VEGF) and erythropoietin (Epo) expression, and alleviates the neurological deficit caused by ischemic stroke as well as edema. ġ0-week-old 115 C57BL/6J male mice of weight 27 ± 2 g were randomly divided into four groups: Normal ( n = 27) with no additional treatments Control ( n = 30).

Mice were injected with a mixture of saline solution and DMSO 1% (Thermofisher, Netherlands, Cat: TS-20688) intraperitoneally (IP) at a dose of 0.2 mL/20 g body weight. This group represents mice with ET-1 administration ACF ( n = 30).

Mice were treated with ACF (Sigma-Aldrich, China, Cat: A8251) dissolved in PBS with a concentration of 0.5 mmol/L and stored at 4 ☌.

The ACF solution was IP injected at a dose of 1.5 mg/kg body weight, 24 h before stroke and continually for 12 day up to sacrifice.

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